- 1 Nomenclature
- 2 Historical Use of Cassia acutifolia
- 3 Background
- 4 Pharmaceutical Information
- 5 Evidence or the Use of Cassia acutifolia in the Treatment of Epilepesy
- 6 Safety
Historical Use of Cassia acutifolia
Cassia acutifolia in Traditional Chinese Medicine
Chinese Name (pinyin): Fanxieye
Chinese Name :
Common Name :Senna Leaf
Specific Name : Folium sennae
Description : Leaf os cassia acutifolia: Lanceolate or elongated ovate, slightly rolled, apex macronate or slightly convex, base asymmetrical both surface covered with fine short hairs.
Identification : 1.Powder: pale green or yellowish green, crytal fibres numerous, prisms of calcium oxalate 12 - 15µm in diameter, non-glandular hairs, unicellular, 100 - 350µm long, 12 - 25µm in diameterwith thick warty walls. Clusters of calcium oxalate occurring in parenchymatous cells of mesopyll, 9 - 20µm in diameter. Epidermal cells of both surface, polygonal in surface view with straight anticlinal walls, stomata, paracytic, surrounded by 2, sometimes 3 subsidiary cells.2.To 25mg of the powder add 50ml of water and 2ml of hydrochloric acid, heat on a water bath for 15 minutes, allow to cool. Extract with 40ml of ether, separate the ether layer and dehydrate over anhydrous sodium sulfate and filter. Evaporate 5ml of the filtrate to dryness, allow to cool, add 5ml of ammonia TS, a yellow or orange color is produced, changing to purplish red when heated on a water bath for 2 minutes3.To 0.5g of the powder, add 3ml of a mixture solution of equal quantities of ethanol and water, ultrasonicate for 30 minutes, cetrifuge. The supernatant is used as the test solution. Prepare a solution of Folium Sennae reference drug in the same manner as the reference drug solution. Carry out the method for thin layer chromatogaphy (Appendix Vl B), using silica gel G as the coating substance and ethyl -acetate-propanol-water (4:4:3) as the mobile phase. Apply separately in strip 10µl of each of the two solutions to the same plate. After developing and removal of the plate dry it in the air and examine under ultra violret light (365nm), The fluorescent spots in the chromatogram obtained with the test solution correspond in position and color to the spots in the chromatogram obtained with the reference solution. Spray with 20% nitric acid solution, heat for 10 minutes at 120ºC allow to cool, spray again with 5% potassium hydroxide solution in 50% of ethanol, examine under daylight, the spots in the chromatogram obtained with the test solution correspond in position and color to the spots in the chromatogram obtained with the reference drug solution.Foreign matter : not more than 6% (Appendix lX A)Water: Carry out the method for determination of water (Appendix lX H, method 1) not more than 10.0% of water.Assay: Protect from light throughout the assay.Place 0.15g of the fine powder, accurately weighed into a round bottom flask, add 30ml of water, shake thoroughly, wegh, heat under reflux on a water bath for 15 minutes. Allow to cool, wegh and restore the original weight with water, shake well. Centrifuge and transfer accurately 20ml of supernatant liquid to a 150ml separator. Add 0.1ml of hydrochloric acid (2mol/L), extract with 3 quantities, each 15ml of chloroform, allow to separate and discard the chloroform layer. Add 0.1g of sodium bicarbonate to the water layer, shake for 3 minutes. Cetrifuge and transfer accurately 10ml of the supernatant liquid to a 100 ml round bottom flask. Add 20ml of 10.5% ferric chloride solution, mix well, heat under reflux in a water bath for 20 minutes. Add 1ml of hydrochloric acid again and continue the heating for 20 minutes with frequent shaking until the precipitate is dissolved completely. Allow to cool, transfer the mixture to a separator, extract with 3 quantities each 25ml of ether (previously used to rinse the flask). Wash the combined ether extract with 2 quantitis each 15ml of water and discard the water layer. Filter the ether extract through dry filter paper to a 100 ml amber colored volumetric flask and wash the filter with ether. Combine washings with ether extracts then add ether to volume. Measure accurately 10ml of the ether extracts, evaporate carefully to dryness, dissolve the residue in 10ml 0f 0.5% magnesium acetate in methanol, accurately measured. Carry out the methiod for spectrophotometry (Appendix V B), perform methanol as a blank, Immediately measure the absorbance at 515nm. Calculate the content of sennoside B, taking 240as the value of A (1%, 1cm). It contains not less than 2.5% of total sennosides, calculated as sennoside B.
Action : To purge away heat, relax the bowels and induce diuresis.
Indication : accumulation of heat marked by constipation and abdominal pain; edema
Precautions : Used with caution in pregnancy.
Dosage : 2 to 6 g.
Storage : Preserve in a ventilated dry place, protected from light.
Synonymns for Cassia acutifolia
Patent Medicines and Medicines with Multiple Ingredients that include Cassia acutifolia
Evidence or the Use of Cassia acutifolia in the Treatment of Epilepesy
Cohort, Case-Control and Non-Randomized Trials
Randomized Controlled Trials
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